• We can do the following In vivo Animal Model

  • In vivo mouse and rat model for hemorrhagic stroke:

    • To simulate hemorrhagic stroke in mice and rats and to study the novel drugs to rescue the neuronal damage and improve the functional recovery.
    • Autologous blood infusion and collagenase model of hemorrhagic stroke.

    Behavioral Assays:

    • To assess the functional (motor or sensory) recovery following treatment in the autologous blood infusion and collagenase model of hemorrhagic stroke.
    • Rotarod analysis
    • Fore limb placement analysis
    • 30º turn analysis
    • Cylinder task
    • Dot removal task

    Histology Techniques

    • Transcardiac perfusion and cryosectioning of rat and mouse brain
    • Immunohistochemistry
    • Measurement of Stroke volume using the Ziess software.
    • Neuronal, glial cell counts by stereology.

    Tissue culture

    • Preparation of primary cortical neurons and astroyctes from rat embryo brain
    • Long term maintenance of mouse hippocampal cell line, striatal cell line.

    Alzheimer’s disease animal modeling:

    • Rodent model for Alzheimer’s disease ((Mice overexpressing double-mutated form of human APP 695, the Swedish (KM670/671NL) and Indiana (V717F) mutations)
    • Rodent model for mild impairment of oxidative metabolism (Mice model: Pyrithiamine)

    Behavioral Assays:
    • Rotarod analysis: To test the effects of drugs, brain damage, or diseases on motor coordination in rodents.

    Brain microdissection techniques

    • microdissection of rodent brain nuclei
    • micropunch of submedial thalamus nuclei micropunch of substantia nigra (Cell bodies of dopamine neurons)
    • micropunch of nucleus caudate putamen (terminals of dopamine neurons)

    In vivo Parkinson’s disease animal modeling:

    • Mouse model for Parkinson’s disease: MPTP model
    • Rat model for Parkinson’s disease: MPP+ infusion model
    • Rat model for Parkinson’s disease: rotenone infusion model
    • Rat model for Parkinson’s disease: 6-OHDA infusion model

    Rodent Surgery techniques:

    • Rat brain streotaxic surgery
    • Brain microdialysis

    Neurotransmitter analysis:

    • Dopamine, DOPAC, Serotonin, Norephinephrine by High performance liquid chromatography (HPLC-ECD)

    Behavioral Assays:

    • Rotarod analysis: To test the effects of drugs, brain damage, or diseases on motor coordination in rodents.
    • Apomorphine induced rotational studies: As an index of both lesion deficits and drug induced recovery.
    • Amphetamine induced rotational studies: As an index of both lesion deficits and drug induced recovery.
    • Analysis of Exploratory and Motor activity (Open field test and Rota-rod test)
    • Antinociceptive and Anti-inflammatory activity (Hot plate, tail-flick, carageenin-induce)
    • Anti-convulsive activity (Maximal electroshock seizure test, Pentetrazole induced convulsions).

    Drug screening for Parkinson’s disease:

    • Test the efficacy of the following drugs in Parkinson’s models
    • Antioxidants (melatonin)
    • Nonsteriodal anti-inflammatory drugs (Aspirin)
    • Nonsteriodal anti-inflammatory drugs (Acetaminophen)
    • Nonsteriodal anti-inflammatory drugs (Ibuprofen)
    • Neuronal Nitric oxide synthase inhibitors (7-Nitroindazole)
    • Novel bioactive compounds (alkaloids, Steriods, Triterpinoids)

    Intracerebral Hemorrhage Model

    In vitro model for hemorrhagic stroke:

    • To simulate hemorrhagic stroke in in vitro (dish) and identify the therapeutic target and study the novel drugs to rescue the neuronal damage.

    Small animal imaging:

    • To assess the pharmacokinetics of the drugs and to identify the target in vivo.
    In vivo bioluminescence imaging (IVIS) (noninvasive biophotonic and fluorescence rodent imaging method). IVIS is a procedure where gene expression can be examined in live, intact mice.